ELISA principals and applications for professionals
ELISA principals and applications for professionals, ELISA fundementals.
Enzyme-linked immunosorbent Assay (ELISA)
ELISA from fundamentals of immune system and the history of the
analytical assay prior to the invention of ELISA to the materials of choice for
fabrication of the platforms, possible biomolecular interactions, different protocols,
and evaluation parameters among the rest. This book guides Readers through different steps of the analytical assay while familiarizing them with the possible
sources of error in the assay. The course offers detailed insights on the immobilization techniques used for protein attachment, different methods for evaluation
of the assay and calculation of the key important parameters in the analytical assay
such as sensitivity, specificity, accuracy, and limit of detection. Advantages and
shortages of the conventional ELISA as well as different attempts for improvement
of its performance are covered in this book. Merging and intergrading different
technologies with widely known ELISA have opened numerous windows of
opportunity to the advancement of this immunoassay. In that respect, the current
book provides the latest updates on integrated platforms such as ELISpot, plasmonic ELISA, sphere-/bead-based ELISAs, paper-/fiber-based ELISAs as well as
ELISA in micro-devices.
Fundamentals and History of ELISA
Evolution of the Immunoassays Until Invention of ELISA
Principles of the Immune System
Biomolecular Interactions Between Antibody and Antigen . . .
General Overviews on Applications of ELISA . . .
Step by Step with ELISA: Mechanism of Operation, Crucial
Elements, Different Protocols, and Insights on Immobilization
and Detection of Various Biomolecular Entities . . . . . . . . .
Evaluation of the Detection Results Obtained from ELISA . . .
